Evaluation of ViroTrack Sero Zika IgG/IgM, a New Rapid and Quantitative Zika Serological Diagnostic Assay

Abstract: Dengue virus (DENV) and Zika virus (ZIKV) belong to the flavivirus genus and are
antigenically closely related. They also share the same mosquito vector and can cause similar
symptoms upon infection. However, DENV and ZIKV infections lead to different clinical sequelae
and treatments; therefore, clinicians need rapid and accurate diagnostics capable of distinguishing
between the two diseases. Methods: We employed the immuno-magnetic assay technology on a
microfluidic cartridge (ViroTrack Sero Zika IgG/IgM) for diagnosis of ZIKV infection based on the
aggregation of magnetic nanoparticles. We carried out three serological studies including samples
from the Dominican Republic, USA, and Nicaragua, aimed at detecting ZIKV-specific IgG and IgM
using the ViroTrack Sero Zika IgG/IgM test. Results: The seroconversion results were comparable
with ZIKV IgG and IgM reactivity measured by the commercial ZIKV ELISA kit. The sensitivity and
specificity for both ZIKV IgG and IgM tested by the ViroTrack Sero Zika IgG/IgM was approximately
98% and 93%, respectively. Conclusion: Serological detection of ZIKV infection by the new ViroTrack
Sero Zika IgG/IgM test shows promising performance and limited cross-reactivity with DENV.

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