Evaluation of a novel microfluidic immunomagnetic agglutination assay method for detection of dengue virus NS1 antigen

Abstract: Dengue virus (DENV) is the most important arbovirus worldwide, causing infections in
endemic countries and returning travellers from these areas. Rapid diagnostic tests are
needed to improve patient management and monitor local transmission. The detection of
DENV non-structural protein 1 (NS1) is a useful tool for the diagnosis, but the currently available methods can be time consuming or lack sensitivity. The objective of our study was to
evaluate a new rapid and semi-quantitative microfluidic DENV NS1 immuno-magnetic
agglutination assay based on aggregation of magnetic nanoparticles detected by an electronic reader (Virotrack Dengue Acute and Blubox, Blusense diagnostics, Copenhagen,
Denmark).

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